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Registro Completo |
Biblioteca(s): |
Embrapa Semiárido. |
Data corrente: |
27/08/2001 |
Data da última atualização: |
09/11/2017 |
Autoria: |
SANTOS, C. A. F. |
Afiliação: |
CARLOS ANTONIO FERNANDES SANTOS, CPATSA. |
Título: |
Biometrical studies and quantitative trait loci associated with major products of the carotenoid pathway of carrot (Daucus carota L.) |
Ano de publicação: |
2001 |
Fonte/Imprenta: |
2001. |
Páginas: |
265 f. |
Idioma: |
Inglês |
Notas: |
Thesis (Doctor of Philosophy) - University of Wisconsin, Madison. |
Conteúdo: |
Orange carrots are a top ranked vegetable in terms ofpro-vitamin A content. Carotenoid pathway products were investigated in F2 populations from two different carrot crosses: orange Brasilia x dark orange HCM and orange B493 x white wild QAL. Broad sense heritabilities va1ues for a1l carotenoids were greater than 90% in the B493 x QAL cross and from 35% to 70% among different carotenoid,s in the Brasilia x HCM cross. The estimated number of factors was 4 for a-carotene, 3 for ~-carotene and total carotenes and one for ?- carotene, lycopene and phytoene in the orange x dark orange cross, and 4 for a-carotene, 1-2 for lycopene and tota1 carotenes and 1 for the other carotenes in the orange x white cross. In comparison to the known biochemical pathways the correct order of substrates and products, phytoene-+? -carotene-+ lycopene, was identified in the path analysis of f3-carotene in the cross Brasilia x HCM but not in the correlation analysis. Linkage grouping ana1ysis assigned 287 and 250 scored molecular markers to the nine chromosomes of carrots, at LOD scores ranging from 3.0 to 7.0 and the average marker spacing was 4.78,4.80,5.54 and 5.13 cM in 11 the Brasilia-, HCM-, B493 and QAL-coupling phase maps, respectively. Interval mapping performed with the orange x dark orange cross detected four, eight, three, one, five and three putative QTL associated with accumulation of ?-carotene, a-carotene, 13-carotene, lycopene, phytoene and total carotenoids, respectively, with major QTL explaining from 10.2 to 13.0% of total phenotypic variation. In the B493 x QAL population single marker analysis identified loci explaining 13.8%,6.8%, 19.3%,5.7%, 17.5% and 20.2% of total phenotypic variation for ?-carotene, a-carotene, 13-carotene, lycopene, phytoene and tota1 carotenoids content, respectively. Overall analysis showed clustered loci affecting the phenotypic variation of carotenoidpathway suggesting clusters of related-pathway loci as an evolutionary mechanism and supporting an adaptive evolutionary model suggested by H. A. Orr. Path analysis and QTL studies suggested that phytoene biosynthesis, perhaps associated with a root specific signal, are the two key factors limiting the carotenoid pathway in roots of white carrots. MenosOrange carrots are a top ranked vegetable in terms ofpro-vitamin A content. Carotenoid pathway products were investigated in F2 populations from two different carrot crosses: orange Brasilia x dark orange HCM and orange B493 x white wild QAL. Broad sense heritabilities va1ues for a1l carotenoids were greater than 90% in the B493 x QAL cross and from 35% to 70% among different carotenoid,s in the Brasilia x HCM cross. The estimated number of factors was 4 for a-carotene, 3 for ~-carotene and total carotenes and one for ?- carotene, lycopene and phytoene in the orange x dark orange cross, and 4 for a-carotene, 1-2 for lycopene and tota1 carotenes and 1 for the other carotenes in the orange x white cross. In comparison to the known biochemical pathways the correct order of substrates and products, phytoene-+? -carotene-+ lycopene, was identified in the path analysis of f3-carotene in the cross Brasilia x HCM but not in the correlation analysis. Linkage grouping ana1ysis assigned 287 and 250 scored molecular markers to the nine chromosomes of carrots, at LOD scores ranging from 3.0 to 7.0 and the average marker spacing was 4.78,4.80,5.54 and 5.13 cM in 11 the Brasilia-, HCM-, B493 and QAL-coupling phase maps, respectively. Interval mapping performed with the orange x dark orange cross detected four, eight, three, one, five and three putative QTL associated with accumulation of ?-carotene, a-carotene, 13-carotene, lycopene, phytoene and total carotenoids, respectively, with major... Mostrar Tudo |
Palavras-Chave: |
AFLP; Carotene; Carotenoid content; Conteudo; Correlacao fenotipa; Dissecacao genetica; Genetic crosses; Genetic dissection; Heranca; Inheritance; Mapeamento; Mapping; Melhoramento genético; Molecular markers. |
Thesagro: |
Carotenóide; Cenoura; Cruzamento; Daucus Carota; DNA; Genética; Marcador Genético; População; Variedade. |
Thesaurus Nal: |
carotenes; carrots; genetic markers; phenotypic correlation; population; varieties. |
Categoria do assunto: |
G Melhoramento Genético |
Marc: |
LEADER 03550nam a2200481 a 4500 001 1134339 005 2017-11-09 008 2001 bl uuuu m 00u1 u #d 100 1 $aSANTOS, C. A. F. 245 $aBiometrical studies and quantitative trait loci associated with major products of the carotenoid pathway of carrot (Daucus carota L.) 260 $a2001.$c2001 300 $a265 f. 500 $aThesis (Doctor of Philosophy) - University of Wisconsin, Madison. 520 $aOrange carrots are a top ranked vegetable in terms ofpro-vitamin A content. Carotenoid pathway products were investigated in F2 populations from two different carrot crosses: orange Brasilia x dark orange HCM and orange B493 x white wild QAL. Broad sense heritabilities va1ues for a1l carotenoids were greater than 90% in the B493 x QAL cross and from 35% to 70% among different carotenoid,s in the Brasilia x HCM cross. The estimated number of factors was 4 for a-carotene, 3 for ~-carotene and total carotenes and one for ?- carotene, lycopene and phytoene in the orange x dark orange cross, and 4 for a-carotene, 1-2 for lycopene and tota1 carotenes and 1 for the other carotenes in the orange x white cross. In comparison to the known biochemical pathways the correct order of substrates and products, phytoene-+? -carotene-+ lycopene, was identified in the path analysis of f3-carotene in the cross Brasilia x HCM but not in the correlation analysis. Linkage grouping ana1ysis assigned 287 and 250 scored molecular markers to the nine chromosomes of carrots, at LOD scores ranging from 3.0 to 7.0 and the average marker spacing was 4.78,4.80,5.54 and 5.13 cM in 11 the Brasilia-, HCM-, B493 and QAL-coupling phase maps, respectively. Interval mapping performed with the orange x dark orange cross detected four, eight, three, one, five and three putative QTL associated with accumulation of ?-carotene, a-carotene, 13-carotene, lycopene, phytoene and total carotenoids, respectively, with major QTL explaining from 10.2 to 13.0% of total phenotypic variation. In the B493 x QAL population single marker analysis identified loci explaining 13.8%,6.8%, 19.3%,5.7%, 17.5% and 20.2% of total phenotypic variation for ?-carotene, a-carotene, 13-carotene, lycopene, phytoene and tota1 carotenoids content, respectively. Overall analysis showed clustered loci affecting the phenotypic variation of carotenoidpathway suggesting clusters of related-pathway loci as an evolutionary mechanism and supporting an adaptive evolutionary model suggested by H. A. Orr. Path analysis and QTL studies suggested that phytoene biosynthesis, perhaps associated with a root specific signal, are the two key factors limiting the carotenoid pathway in roots of white carrots. 650 $acarotenes 650 $acarrots 650 $agenetic markers 650 $aphenotypic correlation 650 $apopulation 650 $avarieties 650 $aCarotenóide 650 $aCenoura 650 $aCruzamento 650 $aDaucus Carota 650 $aDNA 650 $aGenética 650 $aMarcador Genético 650 $aPopulação 650 $aVariedade 653 $aAFLP 653 $aCarotene 653 $aCarotenoid content 653 $aConteudo 653 $aCorrelacao fenotipa 653 $aDissecacao genetica 653 $aGenetic crosses 653 $aGenetic dissection 653 $aHeranca 653 $aInheritance 653 $aMapeamento 653 $aMapping 653 $aMelhoramento genético 653 $aMolecular markers
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Embrapa Semiárido (CPATSA) |
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6. | | SANTOS, C. A. F. Dispersao da variabilidade fenotipica do umbuzeiro no Semi-Arido brasileiro. Pesquisa Agropecuaria Brasileira, Brasilia, v.32, n.9, p.923-930, set. 1997. E em Revista Brasileira de Genetica, Sao Bernardo dos Campos, v. 19, n.3, p.287, set. 1996. Suplemento.Tipo: Artigo em Periódico Indexado | Circulação/Nível: Nacional - B |
Biblioteca(s): Embrapa Semiárido; Embrapa Unidades Centrais. |
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20. | | SANTOS, C. A. F. Recursos genéticos e pré-melhoramento do umbuzeiro. In: LEDERMAN, I. E.; LIRA JÚNIOR, J. S. de; SILVA JÚNIOR, J. F. da (Ed.). Spondias no Brasil: umbu, cajá e espécies afins. Recife: IPA; Embrapa Agroindústria Tropical; UFRPE, 2008. p. 91-96.Tipo: Capítulo em Livro Técnico-Científico |
Biblioteca(s): Embrapa Semiárido. |
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Registros recuperados : 714 | |
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